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目的:研究脂氧素A4在睾丸缺血-再灌注损伤过程中对免疫细胞CD4~+ T的调节,保护生殖细胞功能作用。方法:健康成年大鼠40只随机分成A组(假手术组)、B组(睾丸扭转组)、C组(扭转复位组)、D组(扭转复位脂氧素A4治疗组),每组各10只。于术后24h分别检测患侧睾丸组织中IFN-γ、IL-2、IL-4、IL-10的浓度,以及睾丸组织中MDA、SOD、GPx含量。对各组睾丸组织行生殖细胞凋亡检测,行HE染色和Johnsen功能评分等形态学观察。结果:与A组相比,睾丸扭转后细胞因子IFN-γ、IL-2和组织中MDA显著升高(P<0.05),而SOD、GPx明显降低。与B和C组比较,D组细胞因子IL-4、IL-10,以及组织中SOD、GPx显著升高(P<0.05),细胞因子IFN-γ、IL-2和睾丸组织MDA水平降低明显。与B和C组比较,D组睾丸凋亡指数明显降低(P<0.05),Johnsen评分明显升高(P<0.05)。透视电镜下,B组和C组生殖细胞内线粒体内嵴结构破坏,溶酶体肿胀,细胞间隙增宽,D组细胞结构完整。结论:在睾丸缺血-再灌注过程中LXA4可以通过调节CD4~+ T细胞炎性因子的释放,减轻再灌注损伤炎症对生殖细胞的损伤,对生殖功能起保护作用。
OBJECTIVE: To study the regulation of CD4 ~ + T in the immune cells during the testicular ischemia-reperfusion injury and to protect the function of germ cells by lipoxin A4. Methods: Forty healthy adult rats were randomly divided into three groups: A group (sham operation group), B group (testicular torsion group), C group (TCD group) and D group 10 only The concentrations of IFN-γ, IL-2, IL-4 and IL-10 in the ipsilateral testis and the levels of MDA, SOD and GPx in the testis tissue were detected 24h after operation respectively. Apoptosis of testis in each group of test cells were detected by HE staining and Johnsen score and other morphological observation. Results: Compared with group A, the levels of cytokines IFN-γ, IL-2 and MDA in testis were significantly increased (P <0.05) and SOD and GPx were significantly decreased after testicular torsion. Compared with B and C groups, the levels of cytokines IL-4, IL-10, and SOD and GPx in group D were significantly increased (P <0.05), and the levels of cytokines IFN-γ, IL-2 and testicular tissue MDA decreased significantly . Compared with group B and group C, the apoptosis index of testis in group D was significantly lower (P <0.05), Johnsen score was significantly higher (P <0.05). Under electron microscope, the mitochondrial inner ridge structure of germ cells in group B and group C were destroyed, the lysosomes were swollen, the cell gap widened, and the cell structure in group D was intact. CONCLUSION: LXA4 can protect the reproductive function by regulating the release of inflammatory cytokines in the process of testis ischemia-reperfusion.