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目的:观察不同穴位疗法对高强度军训士兵自由基代谢的影响。方法:120名男性士兵随机分为训练对照组(n=30)、穴位电疗组(n=30)、穴位艾灸组(n=30)和穴位注射组(n=30)。除训练对照组外,其余3个干预组士兵,从高强度军事训练前2周开始分别采用穴位电疗(电疗参数为连续波,频率2 Hz,强度20 mA,30 min/次,1次/天)、穴位艾灸(艾条温和灸,30 min/次,1次/天)和穴位注射(生理盐水,1 ml/穴,1/Qod)3种方法刺激双侧足三里穴,连续2周。分别测试各组训练前和训练第1、5天时5 km跑成绩、血浆乳酸(LA)含量、超氧化物歧化酶(SOD)活性和丙二醛(MDA)浓度。结果:训练第1天和第5天,各干预组5 km跑成绩、SOD活力、SOD/MDA比值均显著优于训练对照组(P<0.05,P<0.01),LA含量、MDA浓度均显著低于训练对照组(P<0.01)。训练第5天,穴位电疗组5 km跑成绩显著优于穴位注射组(P<0.01),穴位电疗组与穴位艾灸组SOD活力均显著高于穴位注射组(P<0.05,P<0.01)。四组LA含量、MDA浓度及SOD活力在训练第1天和第5天均显著高于实验前(P<0.01),训练对照组SOD/MDA比值显著低于实验前(P<0.01),各干预组训练前后无明显变化(P>0.05)。结论:针灸疗法能减少高强度军训士兵机体血乳酸堆积,提高抗氧化能力,减少脂质过氧化反应,有效防治运动性疲劳,穴位电疗组疗效略优。
Objective: To observe the effect of different acupoints therapy on free radical metabolism in high-intensity military training soldiers. Methods: 120 male soldiers were randomly divided into training control group (n = 30), electroacupuncture group (n = 30), acupoint moxibustion group (n = 30) and acupoint injection group (n = 30). In addition to training control group, the remaining three intervention group soldiers, respectively, from 2 weeks before the high-intensity military training using acupoint electrotherapy (electrotherapy parameters for the continuous wave, frequency 2 Hz, intensity 20 mA, 30 min / time, 1 time / day ) Acupoint moxibustion (Moxa mild moxibustion, 30 min / time, once a day) and acupoint injection (saline, 1 ml / acupoint, 1 / Qod) . The results of 5 km running, plasma lactate (LA) content, superoxide dismutase (SOD) activity and malondialdehyde (MDA) concentration before and 1.5 and 5 days of training were tested in each group. Results: On the first day and the fifth day of training, the 5 km running scores, SOD activity and SOD / MDA ratio in each intervention group were significantly better than those in training control group (P <0.05, P <0.01), LA content and MDA concentration were significantly Lower than the training control group (P <0.01). On the fifth day of training, the activity of electroacupuncture at 5 km was significantly better than that of acupoint injection (P <0.01), while the activity of SOD at acupuncture points electroacupuncture and acupoint moxibustion was significantly higher than that of acupoint injection (P <0.05, P <0.01) . The content of LA, the content of MDA and the activity of SOD in the four groups were significantly higher than those before training (P <0.01) on the first day and the fifth day, and the SOD / MDA ratio in the training group was significantly lower than that before the training (P <0.01) The intervention group had no significant changes before and after training (P> 0.05). Conclusion: Acupuncture therapy can reduce blood lactic acid accumulation, improve antioxidant capacity, reduce lipid peroxidation and effectively prevent exercise fatigue in high-intensity military soldiers.