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先将合成的纳米镍修饰裸玻碳电极,再在修饰电极表面热聚合一种以甲基丙烯酸为功能单体、马来松香丙烯酸乙二醇酯为交联剂、纳米氧化铜掺杂的异戊巴比妥分子印迹敏感膜。研究了印迹电极的最佳成膜条件及其最佳实验条件。采用循环伏安法(CV)和电化学交流阻抗法(EIS)对印迹传感器的电化学性能进行表征。使用红外光谱和扫描电镜分别探究此印迹敏感膜的结构及表面形貌。在最佳实验条件下,以K3Fe(CN)6为分子探针的差分脉冲伏安法(DPV)峰电流响应值与异戊巴比妥的浓度在6.5×10-8~1.8×10-4mol/L范围内呈现良好的线性关系(线性相关系数R=0.9986);检出限为1.1×10-9mol/L(S/N=3)。传感器能用于猪肉中残留异戊巴比妥的检测,加标回收率在96.5%~103.2%之间。
First, the modified nano-nickel modified bare glassy carbon electrode, and then the modified electrode surface thermal polymerization of a methacrylic acid as a functional monomer, maleic rosin acrylate as a cross-linking agent, nano-copper oxide doped heteropoly Pentobarbital Molecularly Imprinted Sensitive Membrane. The best conditions of film formation and the best experimental conditions were studied. The electrochemical properties of imprinted sensors were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Infrared spectroscopy and scanning electron microscopy were used to explore the structure and surface morphology of the sensitive membrane. Under the best experimental conditions, the peak current response of differential pulse voltammetry (DPV) with K3Fe (CN) 6 as molecular probe and the concentration of amobarbital in the range of 6.5 × 10-8 ~ 1.8 × 10-4mol / L range (linear correlation coefficient R = 0.9986). The detection limit was 1.1 × 10 -9 mol / L (S / N = 3). The sensor can be used for the detection of residual pentobarbital in pork with the recoveries of 96.5% -103.2%.