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目的 研究中国汉族和藏族两个群体中 P基因 3个外显子多态性。方法 利用 PCR- RFL P多态性方法扩增 P基因的 3个外显子 10、14、2 4,对其产物分别用 Hae 、Mbo 、Mae 酶切 ,4%琼脂糖凝胶电泳确定基因型。结果 A35 5 A和 G780 G的等位基因频率在两个群中的差异具有显著性 (χ2 =2 4.5 4,χ2 =37.0 5 ,P<0 .0 0 1) ,各基因型频率分布在两个群体间差异具有显著性 (χ2 =2 8.39,P<0 .0 0 1;χ2 =33.72 ,P<0 .0 0 1) ;在两个群体内部男女基因型频率差异无显著性 ;IVS13- 15的等位基因频率在两个群体中差异无显著性 (χ2 =2 .0 6 ,P>0 .0 5 )。汉族与藏族的基因型分布符合 Hardy- Weinberg平衡。结论 P基因在不同的人群中具有很高的多态性
Objective To study the polymorphism of three exons of P gene in Chinese Han and Tibetan populations. Methods Three exons 10, 14, and 24 of P gene were amplified by PCR-RFLP polymorphism and their products were digested with Hae, Mbo and Mae respectively and genotypes were determined by 4% agarose gel electrophoresis . Results The allele frequencies of A35 5 A and G780 G were significantly different between the two groups (χ2 = 2 4.54, χ2 = 37.0 5, P <0.01) There was no significant difference in genotype frequencies between male and female in two groups (χ2 = 2 8.39, P <0.01; χ2 = 33.72, P <0.001) Allele frequency of 15 was not significantly different between the two populations (χ2 = 2.06, P> 0.05). Han and Tibetan genotype distribution in line with the Hardy-Weinberg equilibrium. Conclusion The P gene has high polymorphism among different populations