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目的:建立孕期家装污染大鼠模型,并探讨其对孕鼠子代神经元形态和神经行为的影响。方法:将22只成年孕鼠随机分为对照组和染毒组,分别在孕早期(D1-D10)给予相应的处理。在生后不同时间观察新生大鼠大脑的发育并检测脑组织重量,同时观察新生大鼠皮层神经元的形态变化并检测学习记忆活动。结果:各组新生大鼠脑组织无畸形,且各组大鼠脑组织重量无显著变化,但与对照组相比染毒组皮层神经元的排列及形态欠规则。出生一月后染毒组皮层神经元突起的数量及长度均少于对照组。行为学检测观察到染毒组大鼠其子代大鼠的学习记忆能力明显低于对照组。结论:家装污染物可导致新生大鼠海马神经元的形态学改变并抑制神经元突起的发育与生长,对大鼠的学习记忆功能有明显的损害作用。
OBJECTIVE: To establish a rat model of domesticated pollution during pregnancy and investigate its effects on neuronal morphology and neurobehavioral behavior in progeny of pregnant rats. Methods: Twenty-two adult pregnant rats were randomly divided into control group and exposure group. The corresponding treatment was given in early pregnancy (D1-D10). The growth of newborn rat’s brain was observed at different times after birth and the weight of brain tissue was measured. Meanwhile, the morphological changes of cortical neurons in newborn rats were observed and the learning and memory activities were measured. Results: There was no deformity in the brain of neonatal rats in each group, and the weight of brain tissue in each group showed no significant change. However, compared with the control group, the arrangement and morphology of neurons in the cortex were not regular. The number and length of neurites in cortical neurons in the exposed group were less than those in the control group after birth. Behavioral tests showed that the learning and memory abilities of their offspring rats were significantly lower than those of the control group. Conclusion: Home-made pollutants can cause morphological changes of hippocampal neurons in neonatal rats and inhibit neurite outgrowth development and growth, and have obvious damaging effects on learning and memory in rats.