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报道在裸鼠体内建立人胃原位癌模型的结果。裸鼠经麻醉后,在近前胃处的胃粘膜下接种人胃癌细胞SGC-790l悬液0.02ml(含癌细胞约10~6)。种后三天可见接种部位苍白、僵硬,种后一周t,胃粘膜接种部位隆起小结节并向周围浸润。种后二周,胃部隆起肿块明显,胃粘膜皱襞消失,局部淋巴结肿大,腹腔内可见少量腹水。种后三周,胃部肿块增大质硬,与周围结肠、脾脏等粘连成团块状,腹壁及大网膜可见散在小球形癌灶,腹腔内有明显血性腹水,荷瘤鼠呈现蛙形腹、恶液质。存活期为16～25天,局部肿瘤直径超过1cm,病理证实癌细胞在粘膜与肌层浸润性生长并累及浆膜。接种后第八天注射~(125)I标记的胃癌单克隆抗体MGB_2(~(125)I-MGB_2)进行生物学分布研究。注射四天后肿瘤/非瘤组织的放射性比值(T/NT):血2.01,肝3.57,脾,3.98,肺3.67,正常胃4.11。SPECT放射免疫显像可见肿瘤局部放射性浓聚。上述结果表明:已建立的人胃癌裸鼠胃原位接种模型,再现了临床胃癌的局部浸润、淋巴结转移、腹腔内播散等病理过程,为胃癌研究提供了一种新的与人体更为接近的动物模型。我们用此模型对临床常用抗胃癌药物进行实验治疗研究:在裸鼠胃原位接种PSGC-7901后,分别予以每周一次或两次的给药治疗。连续给药三周,以荷瘤鼠存活期评价疗效。结果表明,以MMC的疗效为最好,治疗组小鼠与对照组比较,存活时间延长90％以上,其次为cis-DDP,而5-FU及CTX的效果较差,Me-CCNU则无效。
Reported in nude mice in vivo model of human gastric cancer results. Nude mice after anesthesia, gastric mucosa near the stomach before inoculation of gastric cancer cells SGC-790l suspension 0.02ml (containing cancer cells about 10 to 6). Vaccination site visible after three days of pale, stiff, one week after planting, gastric mucosal uplift of small parts of the site uplift and infiltration around. Two weeks after planting, the bulge of the stomach was obvious, the folds of gastric mucosa disappeared, the local lymph nodes were swollen, and a small amount of ascites was seen in the abdominal cavity. Three weeks after planting, the mass of the stomach mass increased hard and hard, with the surrounding colon, spleen and other adhesions into a clumpy, abdomen and omentum scattered scattered small spherical foci, abdominal bloody significant ascites, tumor-bearing mice showed frog Abdomen, evil liquid. Survival of 16 to 25 days, the local tumor diameter of more than 1cm, pathological confirmed cancer cells in the mucosa and muscle invasive growth and involving the serosa. On the eighth day after inoculation, the (125) I-labeled gastric cancer monoclonal antibody MGB_2 (~ (125) I-MGB_2) was injected into the biodistribution study. Tumor / non-tumor tissue radioactivity ratio (T / NT) four days after injection: blood 2.01, liver 3.57, spleen, 3.98, lung 3.67, normal stomach 4.11. SPECT radioimmunoimaging shows tumor local radioactive concentration. The above results show that the in situ inoculation model of human gastric cancer in nude mice has reproduced the pathological process of local gastric cancer such as local infiltration, lymph node metastasis and intraperitoneal dissemination, and has provided a new kind of gastric cancer closer to the human body Animal model. We use this model for clinical trials of anti-cancer drugs commonly used in experimental treatment: in situ inoculated with nude mice PSGC-7901, were given once or twice a week treatment. Continuous administration for three weeks to evaluate the survival of tumor-bearing mice efficacy. The results showed that the best effect of MMC, the treatment group mice compared with the control group, the survival time of more than 90%, followed by cis-DDP, and 5-FU and CTX less effective, Me-CCNU is invalid.