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目的:探讨姜黄素对糖尿病大鼠的心脏保护作用及作用机制。方法:通过腹腔注射链脲佐菌素诱导大鼠2型糖尿病。观察姜黄素对大鼠大动脉血流动力学、心脏功能、血糖、血脂、磷脂酰肌醇3-激酶(PI3K)、蛋白激酶B(AKT)、磷酸化蛋白激酶B(p-AKT)、丙二醛(MDA)和一氧化氮(NO)表达,以及过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)、超氧化物歧化酶(SOD)和一氧化氮合酶(NOS)活性的影响。结果:姜黄素处理对糖尿病大鼠AKT表达无明显影响,但可降低血糖、血脂、MDA表达以及血流动力学变化,增加心脏收缩和舒张功能,促进PI3K、p-AKT、NO的表达,提升CAT、GPX、SOD和NOS的活性。结论:姜黄素可通过改善代谢紊乱和血流动力学变化而对糖尿病大鼠心脏具有保护功效,其作用机制与激活PI3K/AKT信号通路而抑制氧化应激和促进NO生成相关。
Objective: To explore the cardioprotective effect and mechanism of curcumin on diabetic rats. Methods: Type 2 diabetes was induced in rats by intraperitoneal injection of streptozotocin. The effects of curcumin on the arterial hemodynamics, cardiac function, blood glucose, blood lipid, PI3K, AKT, p-AKT, (MDA) and nitric oxide (NO), as well as catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD) and nitric oxide synthase ) Activity. Results: Curcumin treatment had no effect on the AKT expression in diabetic rats, but decreased the expression of blood glucose, lipids, MDA and hemodynamics, increased systolic and diastolic function, promoted the expression of PI3K, p-AKT and NO, CAT, GPX, SOD and NOS activity. CONCLUSION: Curcumin can protect the heart of diabetic rats through the improvement of metabolic disorders and hemodynamic changes. Its mechanism of action is related to the activation of PI3K / AKT signaling pathway to inhibit oxidative stress and promote NO production.