羊水细胞培养及染色体制备是染色体病产前诊断的主要手段。由于羊水细胞培养一直存在技术要求高,难度大,培养时间长,易污染,分裂相较少等问题,是许多单位开展产前诊断的难点。近年来,我们在传统的羊水细胞培养技术基础上进行不断探索改进,建立了一种操作简便、快速、稳定、成功率高的羊 Amniotic fluid cell culture and chromosome preparation is the main means of prenatal diagnosis of chromosomal disease. As amniotic fluid cell culture there are always high technical requirements, difficult, long training time, easy to pollution, less split and so on, is the difficulty of many units to carry out prenatal diagnosis. In recent years, we continue to explore and improve on the basis of traditional amniotic fluid cell culture technology, and establish a sheep that is easy to operate, fast, stable and has a high success rate