目的:探索金钗石斛多糖(NDP)对脂多糖(LPS)作用的新生大鼠大脑皮层胶质细胞-神经元混合培养体系的保护作用。方法:制备新生大鼠大脑皮层胶质细胞-神经元混合培养体系,NDP预处理后LPS刺激,观察细胞生长情况并采用Western blot法检测体系中炎症相关因子IL~(-1)β,TNF-α,COX-2和IL-6的蛋白表达及相关调控信号p-NF-κB,p-p38,p-JNK和p-ERK的水平。结果:NDP作用于LPS刺激的大鼠皮层胶质细胞-神经元混合培养体系后,较单用LPS的模型组相比炎症相关因子IL~(-1)β,TNF-α,COX-2和IL-6的蛋白表达及相关调控信号p-NF-κB,p-p38,p-JNK和p-ERK的水平明显降低。结论:NDP可抑制LPS诱导的新生大鼠皮层胶质细胞-神经元混合培养体系炎症相关因子的产生。 Objective: To explore the protective effect of Dendrobium nobile polysaccharide (NDP) on glial cell neuron culture in neonatal rats with lipopolysaccharide (LPS). Methods: Cultured neonatal rat cortical glial cells - neurons mixed culture system, NPS pretreatment LPS stimulation to observe the cell growth and Western blot detection of inflammatory cytokines in the system of IL - (-1) beta, TNF- α, COX-2 and IL-6 protein levels and the related regulatory signals of p-NF-κB, p-p38, p-JNK and p-ERK. Results: Compared with the LPS alone group, NDP could significantly decrease the levels of IL-1β, TNF-α, COX-2 and TNF-α in LPS-stimulated rat cortical neurons mixed culture system IL-6 protein expression and related regulatory signals p-NF-κB, p-p38, p-JNK and p-ERK levels were significantly lower. Conclusion: NDP can inhibit the LPS-induced neonatal rat cortical neurons mixed culture system inflammation-related factors.