[目的]观察妇女妊娠前后血清中氟化物变化.[方法]用离子选择电极,以标准曲线法、标准加入法检测孕妇血清中氟,并与未孕妇女血清进行了比对实验.[结果]本方法线性范围,氟离子浓度0.02-5.0mg／L,r=0.9999,6=59.7,(25℃),检出下限0.012mg／L,孕妇血清和当地未孕妇女血清氟含量分别为0.035±0.006mg/L,0.038±0.008mg／L,P<0.01,具有显著性差异.方法相对标准偏差在5.4％以下,回收率为96％-106％.[结论]本测定方法准确,灵敏,简便;妊娠妇女应注意孕期中的营养补充. [Objective] To observe the changes of fluoride in serum before and after pregnancy. [Methods] The ion-selective electrode was used to detect fluoride in serum of pregnant women by standard curve method and standard addition method. The linear range of this method was fluoride concentration of 0.02-5.0 mg / L, r = 0.9999, 6 = 59.7, (25 ℃), and the detection limit of 0.012 mg / L. The serum fluoride levels of pregnant women and non-pregnant women were 0.035 ± 0.006mg / L, 0.038 ± 0.008mg / L, P <0.01, with significant difference. The relative standard deviation was below 5.4% and the recovery was 96% -106%. [Conclusion] The method was accurate, sensitive and simple Pregnant women should pay attention to nutritional supplements during pregnancy.