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探讨烹调油烟中大气细颗粒物PM2.5(FPCOF)对人肺癌上皮细胞(A-549细胞)抗炎因子IL-10分泌的影响。分别用不同浓度的FPCOF对细胞进行染毒,于12 h、24 h和36 h后用MTT法测细胞存活率;用酶联免疫吸附法测染毒24 h和36 h的细胞上清中IL-10的浓度。结果 :(1)FPCOF作用于A-549细胞24 h和36 h后,细胞存活率随染毒浓度升高而降低,100μg/ml PM2.5+10 mmol/L NAC组存活率高于100μg/ml PM2.5染毒组。(2)FPCOF对A-549细胞作用24 h后,对抗炎因子IL-10的影响不明显;作用36 h后均可见IL-10水平增高,100μg/ml PM2.5+10mmol/L NAC组IL-10浓度低于100μg/ml PM2.5染毒组。提示FPCOF促进A-549细胞中抗炎因子IL-10分泌,其表达要迟于FPCOF对细胞增殖的抑制作用,抗氧化剂NAC对A-549细胞有一定的保护作用。
To investigate the effect of fine particulate matter PM2.5 (FPCOF) on the secretion of anti-inflammatory cytokine IL-10 from human lung cancer epithelial cells (A-549) in cooking fume. The cells were exposed to different concentrations of FPCOF. Cell viability was measured by MTT assay after 12 h, 24 h and 36 h. IL-6, IL- -10 concentration. Results: (1) The survival rate of A-549 cells treated with FPCOF for 24 h and 36 h decreased with the increase of exposure concentration. The survival rate of 100 μg / ml PM2.5 + 10 mmol / L NAC group was higher than 100 μg / ml PM2.5 exposure group. (2) The effect of FPCOF on anti-inflammatory cytokine IL-10 was not obvious after treated with FPCOF for 24 h. The level of IL-10 increased after 36 h treatment, and the concentration of PM2.5 + 10 mmol / L NAC IL-10 concentration was lower than 100μg / ml PM2.5 exposure group. It is suggested that FPCOF can promote the secretion of anti-inflammatory cytokine IL-10 in A-549 cells, and the expression of anti-inflammatory cytokine IL-10 is later than that of FPCOF. Antioxidant NAC has a protective effect on A-549 cells.