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目的:研究碱基切除修复基因XRCC1多态性与肺癌易感性的关系。方法:采用病例-对照研究,收集太原市原发性肺癌患者111例为病例组,同时随机抽取210名健康居民作为对照组,并进行流行病学调查。应用PCR-RFLP方法分析由内切酶MspI识别XRCC1基因Arg399Gln位点的多态性,比较不同基因型与肺癌易感性的关系,以及基因多态性与吸烟之间对肺癌易感性的交互作用。结果:XRCC1密码子399杂合基因型Arg/Gln可能对鳞癌有较弱的保护效应,并可能降低吸烟者患肺癌的危险性。而纯合突变基因型Gln/Gln与和吸烟的存在协同作用可显著提高肺癌的危险度。结论:碱基切除修复基因XRCC1密码子399的多态性可能会对肺癌易感性产生影响,并可能与吸烟量之间存在一定的协同作用。
Objective: To investigate the relationship between XRCC1 polymorphism of base excision repair gene and lung cancer susceptibility. Methods: A case-control study was conducted in 111 patients with primary lung cancer in Taiyuan City, and 210 healthy residents were randomly selected as the control group and epidemiological investigation was conducted. PCR-RFLP method was used to analyze the polymorphism of Arg399Gln site of XRCC1 gene by restriction endonuclease MspI. The relationship between different genotypes and susceptibility to lung cancer and the interaction between gene polymorphism and smoking on lung cancer susceptibility were compared. Results: Arg / Gln genotype of codon 399 of XRCC1 may have a weak protective effect on squamous cell carcinoma and may reduce the risk of lung cancer in smokers. The homozygous mutant genotype Gln / Gln synergistic with the presence of smoking can significantly increase the risk of lung cancer. CONCLUSION: Polymorphism of base excision repair gene XRCC1 codon 399 may have an impact on susceptibility to lung cancer and may have some synergistic effect with the smoking amount.