本文报道用GLC 法同时测定血清中最常用的局部麻醉和麻醉药。实验前,先进行试样的提取,即在1ml 血清试样中加入5mg/L 的内标——丙胺卡因(Prilocaine)标准溶液100μl,5ml 乙醚和1N NaOH 100μl,涡旋混合,离心分离醚层,加1N HCl 500ml 旋混,离心,弃去醚层; This article reports the simultaneous determination of the most commonly used local anesthetics and anesthetics in serum by the GLC method. Before the experiment, the sample was first extracted by adding 5 mg / L of a standard 5 mg / L Prilocaine standard solution to 100 ml of 1 ml serum sample, 5 ml of diethyl ether and 100 μl of 1N NaOH, vortexing and centrifuging the ether Layer, add 1N HCl 500ml screw mixed, centrifuged, the ether layer was discarded;