过去对肌组织和结缔组织的鉴别染色,较常用的方法是苦味酸-酸性复红(即Van Gie-son’s)染色法。该法只能辨认肌组织的存在,对较纤细的肌纤维难以分辨,加上保存时间较短(约数月即自行退色)。我们对肌纤维的染色方法进行了某些改进,发现酸性茜素黄S和酸性茜素蓝B对肌纤维染色效果最佳,现将染色方法和结果报告如下。 In the past, the most commonly used method for the differential staining of muscle tissue and connective tissue is the picric acid-Van Gie-son’s staining. The law can only identify the presence of muscle tissue, more slender muscle fiber difficult to distinguish, coupled with the preservation of a shorter time (about a few months that their own fade). We made some improvements to the staining method of myofibers and found that Acid Alizarin Yellow S and Acid Alizarin Blue B have the best staining results for myofibers. The staining methods and results are reported below.