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目的:建立薏苡仁生物效价检测方法。方法:以肝癌2215细胞为生物模型,应用MTT法建立薏苡仁抑制肝癌2215细胞生物效价检测方法,以系统的方法学考察所建立方法的科学性。结果:在15.62～250.00mg.mL-1范围内,薏苡仁药材剂量的对数与细胞抑制率呈良好的线性关系(r=0.99,P<0.01),方法学考察符合生物效价检测的要求。结论:基于MTT法的薏苡仁抑制肝癌2215细胞生物活性检测方法可以用于薏苡仁药材的质量评价。
Objective: To establish a biological titer test for Coix seed. Methods: The biological assay of hepatocellular carcinoma cell line 2215 was established. MTT assay was used to determine the biological potency of Coix seed in inhibiting the proliferation of hepatocellular carcinoma cell line 2215. The systematic method was used to investigate the scientificity of the established method. Results: In the range of 15.62 ~ 250.00mg.mL-1, there was a good linear relationship between the dose of Yiyiren and the cell inhibition rate (r = 0.99, P <0.01). The methodological investigation met the requirement of biological potency test . Conclusion: The method for detecting the biological activity of Coix seed in inhibiting the proliferation of hepatocellular carcinoma cell line 2215 based on MTT assay can be used to evaluate the quality of Coix seed oil.