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从临床免疫学诊断观察,确有部分肝癌病例可在症状比较隐医的少数肝炎或慢性肝炎患者中发生。这一部分患者,由于临床诊断为肝炎,通常只作肝功和乙型肝炎表面抗原(HBsAg)的检测,常易被误诊和漏诊。因此,探索有效检测方法,提高临床诊断准确率,是十分必要的。1973年我们用高滴度乙型肝炎表面抗原,加入适量胎浸液,免疫家兔,制备成功抗乙型肝炎表面抗原和甲种胎儿球蛋白两用的“双联诊断血清”,随后,用马进行免疫,也获得成功,并摸索出一套相应的检测方法。
From the clinical immunological diagnosis of observation, does have some cases of liver cancer can occur in a minority of patients with symptoms of hepatitis or chronic hepatitis. This part of the patients, because of clinical diagnosis of hepatitis, usually only for the detection of liver function and hepatitis B surface antigen (HBsAg), often easily misdiagnosed and missed diagnosis. Therefore, to explore effective detection methods to improve the accuracy of clinical diagnosis, is very necessary. In 1973, we used high titers of hepatitis B surface antigen and added appropriate amount of fetal immersion to immunize the rabbits to prepare a “double diagnostic serum” that was successfully used for both anti-hepatitis B surface antigen and alpha-fetoprotein. Subsequently, Horse immunization, but also to be successful, and explore a set of appropriate detection methods.