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用纸层析结合显色反应初步鉴定了芥蓝，白菜，菜心劣变种子中的荧光泄漏物质，发现它们均含有Ｒｆ值、显色反应与芥子碱相同的化合物，根据芥子碱在ｐＨ＞１０时转变为黄色，最大吸收波长为３８８ｎｍ的特征，利用光密度测定法检验该类种子活力，并计算两者之间的相关系数，其ｒ分别为－０．９２，－０．８１和－０．８１，实验表明，用光密度（３８８ｎｍ）测定法检测这些种子活力是可行的，并且比荧光观测法灵敏，快速，准确。
Paper chromatography combined with color reaction preliminary identified kale blue cabbage cabbage bad seeds in the fluorescence leakage substances found that they contain Rf value, the same color reaction with sinapine compounds, according to the sinapine in the pH> 10 when the yellow color, the maximum absorption wavelength of 388nm features, the use of optical density determination of seed vigor test, and calculate the correlation coefficient between the two, the r were -0.92, -0.81 and - 0.81. Experiments show that it is feasible to measure the viability of these seeds by the optical density (388nm) assay, which is more sensitive, rapid and accurate than fluorescence.