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目的调查研究广东省广州地区水体中人类杯状病毒(HuCV)的污染情况。方法收集广州地区268份自来水、715份矿泉水、119份生活污水和53份池塘水,用荧光定量PCR方法检测样本中的HuCV及GⅠ、GⅡ型的诺瓦克样病毒(NLVs)和扎幌样病毒(SLVs)。结果生活污水、池塘水、自来水、矿泉水中HuCV总检出率分别为9.24%、11.32%、0、0;生活污水中的GⅠ、GⅡ型的NLVs和SLVs检出率分别为2.52%、7.56%和0;池塘水中的GⅠ、GⅡ型的NLVs和SLVs检出率分别为1.89%、9.43%、0、1.89%;在1例生活污水中检测出GⅠ、GⅡ型NLVs,1例池塘水里同时检测出GⅡ型NLVs、SLVs。结论广州地区生活用水是安全的,但环境水体中存在着HuCV不同基因型的混合污染。
Objective To investigate the pollution of human calicivirus (HuCV) in the water of Guangzhou in Guangdong Province. Methods 268 samples of tap water, 715 samples of mineral water, 119 samples of domestic sewage and 53 samples of pond water were collected from Guangzhou. Fluorescent quantitative PCR was used to detect HuCV, G Ⅰ and GV type Novak’s like viruses (NLVs) and Sapporo Like viruses (SLVs). Results The total detection rates of HuCV in domestic sewage, pond water, tap water and mineral water were 9.24%, 11.32% and 0,0, respectively. The detection rates of GVⅠ, GⅡVLVs and SLVs in domestic sewage were 2.52% and 7.56% And 0 respectively. The detection rates of GⅠ and GⅡ NLVs and SLVs in pond water were 1.89%, 9.43%, 0 and 1.89%, respectively. GⅠ and GⅡ NLVs were detected in one case of domestic sewage and one case of pond water GII-type NLVs and SLVs were detected. Conclusion The domestic water use in Guangzhou is safe, but the mixed pollution of different genotypes of HuCV exists in the environmental water body.