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目的:建立反相高效液相色谱法测定复方鱼腥草片中(R,S)-告依春的含量。方法:采用C18色谱柱,以0.05%磷酸溶液-乙腈(93∶7)为流动相,流速0.8 mL.min-1,检测波长245 nm,柱温30℃。结果:(R,S)-告依春在0.028~0.280 mg.mL-1范围内具有良好的线性关系,r=0.9995;平均回收率为99.7%。结论:该方法可作为复方鱼腥草片的质量控制方法。
Objective: To establish an RP-HPLC method for the determination of (R, S) -derivative in compound Houttuynia cordata Thunb. Methods: The mobile phase consisted of 0.05% phosphoric acid solution - acetonitrile (93: 7) at a flow rate of 0.8 mL · min-1 on a C18 column with a detection wavelength at 245 nm and a column temperature of 30 ℃. Results: There was a good linear relationship between (R, S) -epicin and chun-chun in the range of 0.028-0.280 mg.mL-1, r = 0.9995; the average recovery was 99.7%. Conclusion: This method can be used as a quality control method of compound Houttuynia cordata.