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３５只雄性大鼠喂以含１５％脂肪，分别来源于牛油、豆油、紫草油、鱼油和混合油的饲料。喂养１８０天后取肝脏并用冰冷的生理盐水制成５％的肝匀浆。将暴露于四氯化碳的１ｍｌ５％肝匀浆中分别加入００１ｇ／Ｌ、００５ｇ／Ｌ和０１ｇ／Ｌ的紫草色素，比较加入前后由四氯化碳诱导的氧化产物ＴＢＡｒｓ的光密度值（ＯＤ），并用它反映各组氧化损伤的相对程度。结果表明，鱼油组的ＴＢＡｒｓ显著高于其它各组，其次为紫草油组和混合油组，而牛油组的最低。加入紫草素后，ＴＢＡｒｓ在各剂量组均有不同程度的降低，但是在含多不饱和脂肪酸组，加入高剂量的紫草素后降低的效果显著（Ｐ＜００１），而在饱和脂肪酸组则以中等剂量的紫草素降低氧化损伤的效果较好。
Thirty-five male rats were fed a 15% fat diet derived from tallow, soybean oil, lithospermum oil, fish oil and mixed oil, respectively. After 180 days of feeding, the liver was taken and 5% of liver homogenate was made with cold saline. The 1ml5% liver homogenate exposed to carbon tetrachloride were added 0 01g / L, 0 05g / L and 0 1g / L of lithospermic pigment before and after the addition of carbon tetrachloride-induced oxidation products TBArs value of optical density (OD), and use it to reflect the relative extent of oxidative damage in each group. The results showed that the TBArs in fish oil group was significantly higher than the other groups, followed by lithospermum oil group and mixed oil group, while the lowest in butter group. After addition of shikonin, TBArs decreased in all dosage groups to a certain extent, but decreased significantly with addition of high doses of shikonin in the PUFA group (P <001) Fatty acid group is moderate dose of shikonin to reduce oxidative damage better.