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[Objective] This study aimed to compare four staining methods for proteins of SDS-polyacrylamide gel electrophoresis(SDS-PAGE) and explore a suitable staining method for the antitumor active fraction of P.americana after polyacrylamide gel electrophoresis.[Method] BSA was used as the standard for the comparison of Coomassie brilliant blue staining method,potassium staining method,calcium staining method and silver staining method,on the basis,antitumor active fraction samples of P.americana were used for SDS-PAGE electrophoresis and staining.[Result] The results showed that silver staining method could be accurately,quickly and easily used for SDS-PAGE staining of the antitumor active fraction of P.americana.[Conclusion] This study laid the foundation for exploring the medicinal value of P.americana.
[Objective] This study aims to compare four staining methods for proteins of SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and explore a suitable staining method for the antitumor active fraction of P.americana after polyacrylamide gel electrophoresis. [Method] BSA was used as the standard for the comparison of Coomassie brilliant blue staining method, potassium staining method, calcium staining method and silver staining method, on the basis, antitumor active fraction samples of P. americana were used for SDS-PAGE electrophoresis and staining. [Result] The results showed that silver staining method could be accurately, quickly and easily used for SDS-PAGE staining of the antitumor active fraction of P.americana. [Conclusion] This study laid the foundation for exploring the medicinal value of P.americana.