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脂质体不但可以作为药物传输体系, 而且还作为一种模拟生物膜的简单模型. 近期报道竹红菌素的荧光性质决定于其分子内质子转移, 而分子内质子转移环境敏感特性决定其分子的环境敏感荧光性质. 文中以脂质体为模拟生物膜研究竹红菌乙素(HB)在膜上微环境敏感的荧光性质, 包括生理范围 pH 值变动(6.0~8.0)、胆固醇浓度和离子强度的变化对竹红菌素荧光的影响.发现 HB 的荧光强度对微环境敏感且可被调控. 保持磷酸缓冲液(PBS)中胆固醇和离子强度的浓度不变, 发现脂质体中 HB 的荧光强度在 pH7.4 时有一极大值而在缓冲溶液中 pH7.0 时 HB 的荧光强度有一极小值. 保持 pH 值 7.2 不变, HB 脂质体的荧光强度分别在离子强度为 0.12 mol/kg 和胆固醇浓度为 6 ×10-4mol/L 达到峰值. 对于 HB 的 PBS 溶液来说, 它的荧光强度与离子强度成反比. 这种环境敏感的荧光特性可能用于探测生物组织或生物大分子微环境特征.
Liposomes not only can be used as drug delivery system, but also as a simple model to simulate biofilms.Recently, the fluorescence of hypocrellin is reported to depend on its intramolecular proton transfer, and the intramolecular proton transfer environment-sensitive property determines its molecule The fluorescence properties of hypocrellin (HB) sensitive to the membrane were studied by means of liposomes as simulated biofilms, including physiological pH range (6.0 ~ 8.0), cholesterol concentration and ions Intensity changes on the fluorescence of hypocrellin HB fluorescent intensity was found to be sensitive to the microenvironment and can be controlled to maintain the phosphate buffer (PBS) in the concentration of cholesterol and ionic strength unchanged, found in the liposome HB Fluorescence intensity has a maximal value at pH7.4, while the fluorescence intensity of HB has a minimum at pH7.0 in buffer solution.Under pH 7.2, the fluorescence intensity of HB liposomes is at ionic strength of 0.12 mol / kg and a cholesterol concentration of 6 x 10-4 mol / L. The fluorescence intensity of HB is inversely proportional to the ionic strength for HB in PBS This environmentally sensitive fluorescence property may be used in Measuring biological tissue or a biological macromolecule wherein microenvironment.