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目的分析无创性肢体缺血预处理(IPC)对肢体缺血再灌注损伤的干预效果以及NO和ET-1的变化。方法30只大鼠随机均分为对照组和实验组,对照组未经过缺血预处理,直接止血2、3、4 h;实验组经过缺血预处理后,于第2天分别止血2、3、4 h。依次于恢复肢体血流(再灌注)后1、3、7、14 d时抽取血液检测ET-1、NO的水平。结果大鼠体内NO和ET-1在第1、3、7、14天内呈先递增后递减的趋势,实验组NO在第3天缺血4 h时达最大值,对照组NO在第7天缺血2 h时达高峰;实验组ET-1在第7天缺血3h时达最大值,对照组ET-1在第3天4 h时达最大值,差异均有统计学意义(P<0.05)。实验组NO在大鼠血液中第1、3、7天的含量在缺血2 h和3 h时均显著低于对照组(P<0.01);第14天时,仅缺血2 h时显著低于对照组(P<0.01);实验组ET-1在大鼠血液中第1、3、7天的含量在缺血各时间段时均显著低于对照组(P<0.01);第14天时,仅缺血3 h时显著低于对照组(P<0.01)。结论缺血预处理改变了缺血再灌注损伤后血清NO与ET-1的水平。
Objective To analyze the effects of noninvasive limb ischemic preconditioning (IPC) on limb ischemia-reperfusion injury and the changes of NO and ET-1. Methods Thirty rats were randomly divided into control group and experimental group. The rats in control group were treated with ischemic preconditioning for 2, 3 and 4 hours. After ischemic preconditioning, 3,4 h. The levels of ET-1 and NO in the blood were measured at 1, 3, 7 and 14 days after the resuscitation of limb blood flow (reperfusion). Results The levels of NO and ET-1 in rats were increased firstly and then decreased on the 1st, 3rd, 7th and 14th days. NO in the experimental group reached the maximum at 4 hours after the third day of ischemia, The peak of ET-1 reached the peak at 2 h after ischemia in experimental group, reached the maximum at 3 h after ischemia in experimental group, and reached its maximum at 4 h after 3 d of ET-1 in control group (P < 0.05). The levels of NO in the experimental group on the 1st, 3rd, 7th day were significantly lower than those in the control group at 2 h and 3 h (P <0.01); on the 14th day, NO was only significantly lower at 2 h (P <0.01). The content of ET-1 in the experimental group on the 1st, 3rd, 7th day in the ischemia group was significantly lower than that in the control group (P <0.01) on the 14th day , Only 3 h ischemia was significantly lower than the control group (P <0.01). Conclusion Ischemic preconditioning changes the levels of serum NO and ET-1 after ischemia-reperfusion injury.