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目的探讨肺炎支原体肺炎 (MPP)患儿血清补体溶解免疫复合物能力 (CMSC)及红细胞膜CD3 5变化及其在发病机理中的作用。方法采用免疫比浊法测定血清补体C3 、C4,聚乙二醇沉淀比浊法测定免疫复合物 (IC) ,免疫酶法测定CMSC ,采用酶联法测定红细胞膜CD3 5,对MPP患儿及正常对照儿童进行检测。结果MPP患儿血清C3 、C4、IC含量明显高于对照组 (P <0 .0 1) ,尤以伴有肺外并发症的重症组为明显 ,重症组与轻症组比较 ,差异有显著性 (P <0 .0 5 ) ;而CMSC及CD3 5却明显低于对照组 (P <0 .0 1) ,尤以重症组为明显 ,与轻症组比较 ,差异有显著性 (P <0 .0 5 )。结论MPP患儿CMSC及红细胞膜CD3 5变化在MPP发病机理中起重要作用 ,对判定病情程度有实用价值。
Objective To investigate the changes of serum soluble immunocomplex (CMSC) and erythrocyte membrane CD3 5 in children with Mycoplasma pneumoniae pneumonia (MPP) and its role in pathogenesis. Methods Immune turbidimetric method was used to measure serum complement C3 and C4, and polyethylene glycol precipitation turbidimetry was used to measure immune complex (IC). Enzyme-linked immunosorbent assay (ELISA) was used to detect CMSC. Enzyme-linked immunosorbent assay was used to determine erythrocyte membrane CD3 5. Normal control children for testing. Results The levels of serum C3, C4 and IC in children with MPP were significantly higher than those in the control group (P <0.01), especially in the severe group with extrapulmonary complication, and there was significant difference between the severe group and the mild group (P <0.05). However, the levels of CMSC and CD3 5 were significantly lower than those of the control group (P <0.01), especially in the severe group. There was significant difference between the mild group and the mild group (P < 0 .0 5). Conclusion The changes of CMSC and erythrocyte membrane CD3 5 in MPP children play an important role in the pathogenesis of MPP and have practical value in judging the severity of illness.