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为了探索高效的帕金森病大鼠模型的建立方法,通过黑质致密部(substantia nigra pars compacta,SNc)、中脑腹侧背盖区(ventral tegmental area,VTA)双位点注射和内侧前脑束(medial forebrain bundle,MFB)单位点注射6-羟基多巴胺2种方法建立帕金森病大鼠模型;术后连续4周运用阿朴吗啡旋转试验鉴定模型是否成功,并用免疫组化方法验证大鼠的SNc多巴胺能神经元的损毁程度。结果表明:SNc-VTA组造模后1周成功率为12%,4周后成功率为72%,MFB组造模后1周成功率即达到84%,2周后的成功率则达到92%;采用2种方法均可成功建立帕金森病大鼠模型,但MFB单位点注射法在短时间内具有更高的成功率。
In order to explore a method for establishing an efficient model of Parkinson’s disease in rats, we used two-site injection of substantia nigra pars compacta (SNc), ventral tegmental area (VTA) To establish a rat model of Parkinson’s disease by injecting 6-hydroxydopamine into the medial forebrain bundle (MFB) site. After 4 consecutive weeks, apomorphine rotation test was used to determine whether the model was successful. The rats were also verified by immunohistochemistry The degree of damage to SNc dopaminergic neurons. The results showed that the success rate of SNc-VTA group was 12% after 1 week and 72% after 4 weeks. The success rate of 1 week after model making in MFB group was 84%, and after 2 weeks, the success rate was 92% %. The model of Parkinson’s disease can be successfully established by both methods. However, the single point injection method of MFB has a higher success rate in a short period of time.