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本文介绍一种专一性的生色试剂9-甲氧基吖啶(9-MA),它能与有机结构上的脂肪伯胺反应,生成的有色络合物以分光光度法测定,方法灵敏,准确。利用此反应机理测定环丝氨酸的含量。测定方法:精密量取环丝氨酸水溶液(1.0×10~(-5)～3×10~(-4)mol/L)5ml置10ml容量瓶中,加9-MA乙腈溶液(2×10~(-2)mol/L)5.0ml充分混匀,60℃水浴中震荡60min,冷却至室温,用水稀释至刻度,在438nm波长处测定吸收度,同时做空白对照。
This article describes a specific color reagent 9-methoxy acridine (9-MA), which can react with the organic structure of the primary aliphatic amine, the resulting color complex measured by spectrophotometry, the method is sensitive ,accurate. Use this reaction mechanism to determine the content of cycloserine. Assay Method: Add 5 ml of 10 ml volumetric flask to a 5ml volumetric flask containing precise amount of cyclo-serine aqueous solution (1.0 × 10 -5 to 3 × 10 -4 mol / L), add 9-MA acetonitrile solution (2 × 10 ~ -2) mol / L) 5.0ml mix thoroughly, 60 ° C water bath shock 60min, cooled to room temperature, diluted with water to the mark, absorbance was measured at a wavelength of 438nm, while making a blank control.