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目的:观察重组人骨形态发生蛋白(rhBMP) 2诱导犬自体原位气管移植段软骨再生过程中的剂量效应.方法:4组小鼠气 管移植段各环间软组织中植入rhBMP 2胶原缓释系统,其rhBMP 2含量分别为1,2,5和10mg.将移植段气管原位移植,4wk后 观察标本大体和组织学改变,并将测得的新生软骨面积进行比较.结果:rhBMP 2植入组移植段环间均有不同程度的软骨再生 随植入物中rhBMP 2含量的增加,新生软骨面积分别为:(1768.1±449.0),(2364.1±444.3),(3000.7±488.3),(3188.3± 388.3)pixel.4组新生软骨面积差异有显著性(F=5.246,P<0.01).结论:rhBMP-2诱导气管移植段软骨再生的作用具有剂 量效应.
OBJECTIVE: To observe the dose effect induced by recombinant human bone morphogenetic protein (rhBMP) 2 in the process of cartilage regeneration of autologous tracheal transplantation in dogs.Methods: The rhBMP 2 collagen sustained-release system , The rhBMP 2 contents were 1, 2, 5 and 10 mg, respectively.The orthotopic transplanted tracheal was transplanted into the trachea and the gross and histological changes of the specimens were observed after 4 weeks, and the measured new cartilage area was compared.Results: There were different degrees of cartilage regeneration with the rhBMP 2 content in implants in the group of graft segments. The areas of new cartilage were (1768.1 ± 449.0), (2364.1 ± 444.3), (3000.7 ± 488.3) and (3188.3 ± 388.3) There was a significant difference in cartilage area between PFC group (F = 5.246, P <0.01) .Conclusion: The effect of rhBMP-2 on the cartilage regeneration induced by tracheal transplantation has dose-effect.