本实验选用成年雄性SD大鼠15只,经多聚甲醛灌注固定.通过顶叶,冠状切取2mm厚的脑组织块,冰冻切片20～30μm厚,用还原型尼克酰胺腺嘌呤二核苷酸(NADPH)脱氢酶组织化学染色,采用0.1M磷酸缓冲液pH7.4洗切片3次,入Triton—1000.3%室温下(20℃左右)5’～10’酶组织化学处理,Nitroblue Te-trazc lium 0.1mg/ml+1.0mg/ml NADPH(SIGMA公司),稀释液为0.1M Tris—HCl pH 8.0,37℃孵育60’,效果较好.观察结果:NO合成酶神经元各层均有,以Ⅴ—Ⅵ层最多,其次是Ⅱ—Ⅲ层,最少是Ⅰ怪.通过 In this study, 15 adult male Sprague-Dawley rats were fixed by paraformaldehyde perfusion, and 2 mm thick brain tissue pieces were excised from the parietal and coronal sections. Frozen sections were 20-30 μm thick and treated with reduced nicotinamide adenine dinucleotide NADPH) dehydrogenase histochemical staining, the sections were washed three times with 0.1M phosphate buffer pH7.4, Triton-1000.3% 5 ’~ 10’ enzyme at room temperature (about 20 ℃), Nitroblue Te-trazc lium 0.1mg / ml + 1.0mg / ml NADPH (SIGMA), the dilution was 0.1M Tris-HCl pH8.0, incubate 60 ’at 37 ℃, the effect is better.Results: NO synthase neurons in all layers, V-Ⅵ layer up, followed by Ⅱ-Ⅲ layer, at least I strange through