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慢性免疫性血小板减少性紫癜(CITP)是由于患者体内存在自身的抗血小板抗体与血小板相关抗原结合而引起的一种综合征,已经证实一些CITP患者的自身抗体是抗GPⅡb/Ⅲa复合体和GPⅠb,为了确定其中抗GPⅡb/Ⅲa复合体的自身抗体所识别的抗原位点,作者用四种识别GPⅡb/Ⅲa复合体上不同抗原位点的抗GPⅡb/Ⅲa单克隆抗体,通过放射免疫测定法,观察了这四种单克隆抗体对9例CITP患者自身抗体与血小板抗原结合的封闭能力,从而确定这些CITP患者自身抗体所识别的抗原位点。结果表明这些单克隆抗体对自身抗体与血小板抗原结合有着程度不同的封闭作用,从无作用到完全封闭,提示,
Chronic Immune Thrombocytopenic Purpura (CITP) is a syndrome caused by the presence of its own anti-platelet antibodies in combination with platelet-associated antigens in some patients. It has been demonstrated that some autoantibodies to CITP are anti-GPIIb / IIIa complexes and GPIb In order to determine the antigenic site identified by the autoantibodies against the GPIIb / IIIa complex, the authors used four anti-GPIIb / IIIa monoclonal antibodies that recognize different antigenic sites on the GPIIb / IIIa complex and then detected by radioimmunoassay. We observed the blocking ability of these four kinds of monoclonal antibodies on the binding of autoantibodies to platelet antigens in nine patients with CITP to identify the antigenic sites recognized by autoantibodies in these CITP patients. The results showed that these monoclonal antibodies on the platelet antigen autoantibodies with different degrees of closure, from no effect to complete closure, suggesting that,