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目的:研究妊娠期大鼠接触糖皮质激素(GCs)对子代大鼠海马组织血清和糖皮质激素调节蛋白激酶(SGK)表达的影响,并初步探讨其可能原因。方法:根据大鼠发情周期进行合笼,次晨阴道涂片发现精子作为受孕0.5d。孕鼠随机分为对照组和地塞米松组,每组10只。地塞米松组大鼠在妊娠第14日起皮下注射地塞米松0.1mg/(kg·d),对照组注射等量生理盐水。取出生后1、7和42d的子代大鼠海马组织,采用real-time PCR法检测SGK和糖皮质激素受体(GR)mRNA表达情况。结果:地塞米松组子代出生时体质量明显低于对照组,随后出现追赶性生长;地塞米松组子代在出生后1、7、42d,其海马组织SGK和GR mRNA表达较对照组明显减少(P<0.05)。结论:妊娠晚期接触GCs可使子代大鼠海马SGK和GR的基础表达降低。
OBJECTIVE: To study the effect of glucocorticoid (GCs) on the expression of serum and glucocorticoid-regulated protein kinase (SGK) in the hippocampus of offspring rats during pregnancy and to explore the possible reasons. Methods: According to the rat estrous cycle cage, the next morning vaginal smear sperm found as 0.5d. Pregnant mice were randomly divided into control group and dexamethasone group, 10 rats in each group. Rats in dexamethasone group were subcutaneously injected with 0.1 mg / (kg · d) dexamethasone on the 14th day of pregnancy, and the control group was injected with the same amount of saline. The hippocampus of the offspring rats at 1, 7 and 42 days after birth were taken out and the mRNA expression of SGK and glucocorticoid receptor (GR) was detected by real-time PCR. Results: The body weight of dexamethasone group was significantly lower than that of the control group at birth, and then appeared catch-up growth. The expression of SGK and GR mRNA in the hippocampus of dexamethasone group was higher than that of the control group Significantly reduced (P <0.05). Conclusions: The basal expression of SGK and GR in the hippocampus of offspring rats was decreased after exposure to GCs in late pregnancy.