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目的:探讨特发性胎儿生长受限(IFGR)患者胎盘组织中HIF-1α及caspase-3的表达及在发病机制的作用。方法:选取郑州大学第三附属医院2010年7月～2011年3月剖宫产分娩的IFGR孕妇30例作为实验组,同期因社会因素剖宫产分娩的正常足月孕妇30例作为对照组。采用免疫组化SP法检测两组胎盘组织中HIF-1α、caspase-3的表达。结果:①实验组及对照组的胎盘组织中均有HIF-1α、caspase-3蛋白不同程度的阳性表达。②HIF-1α、caspase-3在胎盘合体滋养细胞上的表达,实验组高于正常对照组,差异有统计学意义(P<0.05)。结论:①胎盘组织中HIF-1α、caspase-3表达增加可能是IFGR发病机制中的关键环节之一。②本结果为临床上应用干预手段预防或治疗IFGR提供依据。
Objective: To investigate the expression of HIF-1α and caspase-3 in the placenta of idiopathic fetal growth restriction (IFGR) patients and its role in pathogenesis. Methods: Totally 30 pregnant women with IFGR delivered by cesarean section in the Third Affiliated Hospital of Zhengzhou University from July 2010 to March 2011 were selected as the experimental group. Thirty normal full-term pregnant women delivered by caesarean section due to social factors were selected as the control group. Immunohistochemical SP method was used to detect the expression of HIF-1α and caspase-3 in placenta of two groups. Results: ① There was positive expression of HIF-1α and caspase-3 protein in placenta of experimental group and control group to varying degrees. ② The expression of HIF-1α and caspase-3 in placental syncytiotrophoblasts was significantly higher in the experimental group than in the normal control group (P <0.05). Conclusion: ① The increase of HIF-1α and caspase-3 in placenta may be one of the key links in the pathogenesis of IFGR. ② The results provide the basis for the prevention or treatment of IFGR by clinical intervention.