药物对人血清白蛋白(HSA)和α_1-酸性糖蛋白(AGP)的相互作用已被广泛研究,但尚无结合位点方面的比较性研究。本文采用荧光法和圆二色性分光光度法(CD)研究了9种安定对HSA和AGP的基本作用模式。经数据处理,药物结合分数计算如下: X=(F_0-F_p)/F_0F_p为含药物溶液中蛋白质荧光强度,F_0为不含药物溶液中蛋白质荧光强度。分数曲线值确定后按Scatchard方程绘制: r/D_f=nK-rKr为每摩尔蛋白质所结合药物的摩尔数,n为 Interactions between drugs on human serum albumin (HSA) and α1-acidic glycoprotein (AGP) have been extensively studied, but there are no comparative studies on binding sites. In this paper, the basic mode of action of 9 kinds of diazepam on HSA and AGP was studied by fluorescence method and circular dichroism spectrophotometry (CD). After data processing, the drug binding fraction is calculated as follows: X = (F_0-F_p) / F_0F_p is the fluorescence intensity of the protein in the drug-containing solution, and F_0 is the fluorescence intensity of the protein in the drug-free solution. The score curve is determined by the Scatchard equation: r / D_f = nK-rKr is the number of moles of drug bound per mole of protein, n is