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建立了人参提取物中苯并(α)芘残留量的高效液相色谱分析方法。样品加水浸泡,用正己烷液液萃取,提取液减压蒸干,C18色谱柱分离,以乙腈-水为流动相,外标法定量。结果表明,苯并(α)芘在1.0~100.0μg/L范围内线性良好,相关系数R为0.999 9,平均加标回收率为85.2%~96.7%,相对标准偏差为7.1%~11.2%。方法的定量限为1.0μg/kg。该方法简单快速、灵敏度高、重现性好,能满足人参提取物中苯并(α)芘残留量定量分析的要求。
A high performance liquid chromatographic method for the determination of benzo (α) pyrene in ginseng extract was established. The sample was soaked in water and extracted with n-hexane. The extract was evaporated to dryness under reduced pressure. The residue was separated on a C18 column. Acetonitrile-water was used as the mobile phase and the external standard method was used. The results showed that the linear range was between 1.0 and 100.0 μg / L, the correlation coefficient R was 0.999 9, the average recoveries were between 85.2% and 96.7%, and the relative standard deviations were between 7.1% and 11.2%. The limit of quantitation was 1.0 μg / kg. The method is simple, rapid, sensitive and reproducible, which can meet the quantitative analysis of benzo (α) pyrene residues in ginseng extract.