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目的 :研究复方半枝莲 (SBC)对二乙基亚硝胺 (DEN)诱发大鼠肝癌的防治作用。方法 :利用免疫组化、流式细胞仪、血清和组织生化等检测方法 ,分别在第 14周和第 2 4周观察 SBC对 DEN诱发的肝癌形成过程的影响。结果 :中药组 14周时大鼠肝脏异型性增生灶明显较模型组少 ,2 4周时形成的肝癌结节小而少 ,模型组、中药组肝癌发生率分别为 75 .0 %、5 0 .0 % ;免疫组化显示中药组大鼠肝组织谷胱甘肽 - S-转移酶胎盘型阳性灶面积明显低于模型组 ;肝组织匀浆上清液谷胱甘肽 - S-转移酶含量以及血清 λ-谷氨酰转移酶、碱性磷酸酶、谷丙转氨酶含量也明显低于模型组 ;流式细胞仪检测结果显示 ,中药组大鼠肝细胞 G0 - G1 期比例下降 ,G2 - M期比例升高。结论 :SBC能抑制癌前病变 ,延缓肝癌的形成 ,降低肝癌发病率 ,其作用机制之一可能为阻滞 G2 - M期细胞进展 ,从而抑制DEN引起的肝细胞的去分化和恶性增殖
Objective: To study the preventive and therapeutic effects of compound Scutellaria barbata (SBC) on rat hepatocarcinoma induced by diethylnitrosamine (DEN). Methods: Immunohistochemistry, flow cytometry, serum and tissue biochemical detection methods were used to observe the effect of SBC on DEN-induced hepatocarcinogenesis at the 14th and the 24th week respectively. Results: At 14 weeks in TCM group, hepatic dysplasia of liver was significantly less than that in model group, with small and small nodules of liver cancer at 24 weeks. The incidence of hepatocellular carcinoma in model group and traditional Chinese medicine group were 75.0% and 50% .0%. Immunohistochemistry showed that the area of glutathione S-transferase positive placenta in liver tissue of rats in traditional Chinese medicine group was significantly lower than that in model group; the content of glutathione S-transferase in liver homogenate supernatant Content and the content of serum λ - glutamyltransferase, alkaline phosphatase and alanine aminotransferase were also significantly lower than those in model group. The result of flow cytometry showed that the percentage of G0 - M phase ratio increased. Conclusion: SBC can inhibit precancerous lesions, delay the formation of hepatocellular carcinoma and reduce the incidence of liver cancer. One of its mechanisms may be to block the cell progression at G2 - M phase and thus inhibit the dedifferentiation and malignant proliferation of hepatocytes induced by DEN