论文部分内容阅读
背景:胃癌的发生是生物、环境、宿主等因素共同作用的结果,宿主遗传因素与幽门螺杆菌(H.pylori)感染后的不同临床结局有关。目的:筛选云南红河州哈尼族彝族H.pylori感染人群的胃癌易感基因,探讨不同基因型和等位基因与H.pylori感染宿主胃癌发病风险的相关性。方法:通过PubMed、CNKI和HapMap数据筛选出12个中国人群胃癌易感相关单核苷酸多态性(SNP)位点,以芯片技术对哈尼族彝族H.pylori感染慢性胃炎和胃癌患者的这些SNP位点进行分型。结果:IL-1β-31C/T和IL-1β-511C/T位点存在完全连锁不平衡,其基因型(P=0.014)和等位基因频率(P=0.049)在胃癌和胃炎组中有显著差异,-511CT/-31CT(OR=2.256,95%CI:1.048～4.855)和-511TT/-31CC(OR=3.312,95%CI:1.462～7.502)基因型胃癌发病风险显著高于-511CC/-31TT基因型。COX-2-899C/G位点基因型频率在胃癌和胃炎组中有显著差异(P=0.033),GG基因型胃癌发病风险显著高于CG基因型(OR=2.796,95%CI:1.053～7.423)。TNF-α-238A/G位点基因型频率在胃癌和胃炎组中有显著差异(P=0.037),AA、AG基因型胃癌发病风险显著高于GG基因型(OR=2.600,95%CI:1.130～5.985)。结论:IL-1β-31C、IL-1β-511T等位基因和COX-2-899GG基因型可增高云南红河州哈尼族彝族H.pylori感染人群的胃癌发病风险,TNF-α-238GG基因型对上述人群的胃癌发生具有保护作用。
Background: The occurrence of gastric cancer is a result of the interaction of biological, environmental and host factors. The genetic factors of the host are related to different clinical outcomes after H. pylori infection. Objective: To screen the susceptibility genes of gastric cancer in Han population of Han nationality in Honghe prefecture of Yunnan province to investigate the relationship between different genotypes and alleles and the risk of gastric cancer in H.pylori-infected hosts. Methods: SNP loci in 12 Chinese people were screened by PubMed, CNKI and HapMap data. Chips were used to detect these SNPs in Hani and Yi people with H.pylori infection in patients with chronic gastritis and gastric cancer SNP site type. RESULTS: There was a complete linkage disequilibrium between the IL-1β-31C / T and IL-1β-511C / T loci, with genotypes (P = 0.014) and allele frequencies (P = 0.049) The risk of gastric cancer with -511CT / -31CT (OR = 2.256,95% CI: 1.048-4.855) and -511TT / -31CC (OR = 3.312,95% CI: 1.462-7.502) was significantly higher than -511CC / -31TT genotype. The genotype frequency of COX-2-899C / G was significantly different between gastric cancer and gastritis group (P = 0.033). The incidence of gastric cancer with GG genotype was significantly higher than CG genotype (OR = 2.796, 95% CI: 1.053 ~ 7.423). The genotype frequency of TNF-α-238A / G locus was significantly different between gastric cancer and gastritis group (P = 0.037). The risk of gastric cancer of AA and AG genotypes was significantly higher than that of GG genotype (OR = 2.600, 95% CI: 1.130 ~ 5.985). CONCLUSION: The genotypes of IL-1β-31C, IL-1β-511T and COX-2-899GG can increase the risk of gastric cancer in Han population of Han nationality in Honghe prefecture. The genotype of TNF-α-238GG The above-mentioned population of gastric cancer has a protective effect.