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在pH4.0～5.0的BR缓冲介质中,赤藓红(Ery)与莫西沙星(MXFX)和加替沙星(GTF)等氟喹诺酮类抗生素(FLQs)相互作用形成1:1离子缔合物,体系反应导致共振瑞利散射(RRS)显著增强并出现新的RRS光谱。两种药物的反应产物具有相似的光谱特征,最大散射波长位于568nm处,并在342nm和378nm处有2个较小的散射峰。在342nm处一定浓度的抗生素与散射增强成正比,两种氟喹诺酮类药物的线性范围分别是0.02～2.7μg/mL(MXFX)和0.06～10.2μg/mL(GTF)。据此建立了测定氟喹诺酮类药物的新方法,已用于胶囊和人尿液中的FLQs测定,并对反应机理和RRS增强的原因作了讨论。
Ery’s interact with fluoroquinolone antibiotics (FLQs) such as moxifloxacin (MXFX) and gatifloxacin (GTF) in a BR buffer at pH 4.0-5.0 to form a 1: 1 ion association System reactions lead to significantly enhanced Resonance Rayleigh scattering (RRS) and new RRS spectra. The reaction products of the two drugs have similar spectral characteristics with the maximum scattering wavelength at 568 nm and two smaller scattering peaks at 342 nm and 378 nm. The concentration of antibiotics at 342 nm was proportional to the increase in scattering. The linear range of the two fluoroquinolones was 0.02 to 2.7 μg / mL (MXFX) and 0.06 to 10.2 μg / mL (GTF), respectively. Therefore, a new method for the determination of fluoroquinolones has been established, which has been used for the determination of FLQs in capsules and human urine. The reaction mechanism and the reasons for the enhancement of RRS are also discussed.