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目的筛选肝癌细胞内HAb18G/CD147信号转导通路中差异表达的关键分子。方法提取SMMC-7721细胞和T7721细胞(SMMC-7721经稳定转染并高表达HAb18G/CD147的肝癌细胞),应用信号转导相关基因芯片筛选两种肝癌细胞内差异表达的关键分子。结果基因芯片结果显示共有13个差异表达的基因,在高表达HAb18G/CD147的T7721细胞系中,表达下调的基因包括:骨形成蛋白(BMP)家族的BMP-2、BMP-5,内皮素1(ET-1),结缔组织生长因子-富含半胱氨酸血管生成诱导物61-肾母细胞瘤过表达基因(CCN)蛋白家族的WISP-2(Wnt1-induced signaling proteins-2/CCN5)和富含半胱氨酸蛋白61(cysteine-rich 61/CCN1),前列腺干细胞抗原(PSCA);表达上调的基因包括:白细胞介素10受体α(IL-10Rα),趋化因子家族的IL-6、IL-8和CXC趋化因子配体2(CXCL2/GROβ),线粒体超氧化物歧化酶2(SOD2),B因子(B factor)和转化生长因子β(TGF-β)诱导基因克隆3(βig-h3)的表达。结论共筛选出HAb18G/CD147信号转导通路相关的13个差异表达基因,这些基因与肝癌细胞免疫微环境重塑、血管形成、增殖、侵袭和迁移等生物学过程相关。
AIM: To screen key molecules differentially expressed in HAb18G / CD147 signal transduction pathway in hepatoma cells. Methods SMMC-7721 cells and T7721 cells (SMMC-7721 stably transfected with hepatoma cells expressing HAb18G / CD147) were extracted. The key molecules differentially expressed in both hepatoma cells were screened by signal transduction-related gene microarray. Results Gene microarray results showed that there were 13 differentially expressed genes. Among T7721 cells that highly expressed HAb18G / CD147, the down-regulated genes included BMP-2, BMP-5, ET-1 (Wnt1-induced signaling proteins-2 / CCN5), a family of connexin tissue growth factor-rich cysteine-induced angiogenesis inducer 61-nephroblastoma overexpression gene (CCN) (Cysteine-rich 61 / CCN1) and prostatic stem cell antigen (PSCA). The upregulated genes include interleukin 10 receptor α (IL-10Rα), chemokine family IL -6, IL-8 and CXCL2 / GROβ, SOD2, B and TGF-β 3 (βig-h3) expression. Conclusions A total of 13 differentially expressed genes related to HAb18G / CD147 signal transduction pathway were screened. These genes were related to the biological processes such as immune microenvironment remodeling, angiogenesis, proliferation, invasion and migration of hepatoma cells.