[Objective] To establish a method to control the quality of bergamot volatile oil. [Methods] GC method was used to determine the limonene content of bergamot volatile oil. [Results]The separation was carried out on a 5% Phenyl Methyl Siloxan column( 30 m × 0. 32 mm × 0. 25 μ m); the detector was FID,and the temperatures of the injection port and detector were 250 ℃ and 280 ℃,respectively; temperature programming started at 65 ℃,holding for 1 min,then increased to 100 ℃ at a rate of 2 ℃·min- 1,kept for 1 min; finally,the column temperature increased to 250 ℃ at a rate of 50 ℃·min- 1,kept for 5 min; the carrier gas was nitrogen with flow rate of 0. 8 m L·min- 1,split radio was 40∶ 1,and injection volume was 1 μL. [Conclusions] The calibration curve had good linearity over the range of 0. 613 1 ~ 30. 654μg( r = 0. 999 9). The average recovery of limonene was 98. 96%. The method is simple,sensitive and accurate,with good repeatability and recovery,and it can be used for the quality control of bergamot volatile oil. [Methods] To establish a method to control the quality of bergamot volatile oil. [Methods] GC method was used to determine the limonene content of bergamot volatile oil. [Results] The separation was carried out on a 5% Phenyl Methyl Siloxan column 30 m × 0.32 mm × 0.25 μ m); the detector was FID, and the temperatures of the injection port and detector were 250 ° C and 280 ° C, respectively; temperature programming started at 65 ° C, holding for 1 min, finally, the column temperature increased to 250 ° C at a rate of 50 ° C · min -1, kept for 5 min; the carrier gas was nitrogen with flow rate of 0. 8 m L · min -1, split radio was 40: 1, and injection volume was 1 μL. [Conclusions] The calibration curve had good linearity over the range of 0. 613 1 ~ 30. 654 μg (r = 0.999 9). The average recovery of limonene was 98. 96%. The method is simple, sensitive and accurate, with good repeatability and recovery, and it can be used for the quality control of bergamot volatile oil.