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采用放射性同位素标记方法,研究了GS和SCS对小鼠大脑突触体~(45)Ca摄取和[~3H]MK-801结合位点的影响。结果表明,GS和SCS 1mg/ml能明显抑制突触体对~(45)Ca摄取;SCS作用显著大于GS,且具有相对较强的高钾刺激依赖性。GS与SCS不同,能明显影响[~3H]MK-801与位点结合。结合三种L-型钙通道阻滞剂的类似实验结果,提示药物的钙阻滞强弱与其对NMDA受体-离子复合物通道影响无关,象二氢吡啶类钙阻滞剂Nit一样,GS的脑缺血保护作用可能涉及兼具钙阻滞和影响NMDA受体-离子复合物通道功能。
The effects of GS and SCS on the uptake of ~(45)Ca and [~3H]MK-801 binding sites in mouse brain synaptosomes were studied using radioisotope labeling methods. The results showed that GS and SCS 1mg/ml could significantly inhibit the uptake of ~(45)Ca by synaptosomes; the effect of SCS was significantly greater than that of GS, and it had relatively strong potassium-dependent stimulation. Unlike GS, GS can significantly affect the binding of [~3H]MK-801 to locus. Combination of three L-type calcium channel blockers similar experimental results, suggesting that the drug’s calcium block strength and its impact on the NMDA receptor-ion complex channel, like dihydropyridine calcium blocker Nit, GS The protective effects of cerebral ischemia may involve calcium block and affect the NMDA receptor-ion complex channel function.