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基于土壤微生物遗传多样性随植被和季节的变化而变化的假设,运用变性梯度凝胶电泳技术(DGGE)检测岩溶区草丛(T)、灌丛(S)、次生林(SF)和原生林(PF)群落演替过程中土壤细菌和真菌群落的遗传多样性及其季节变化。随着地上植被的演替土壤细菌群落具有连续性但优势种群不明显,真菌群落没有连续性但优势种群明显。植被和季节对于细菌和真菌群落的Shannon多样性具有不同程度的显著影响,同时存在显著的植被和季节交互作用。草丛土壤中细菌和真菌群落的Shannon多样性有显著的季节变化(p<0.01);灌丛土壤中仅细菌群落多样性有显著季节变化(p<0.05);而森林土壤中细菌和真菌群落多样性没有显著的季节变化。土壤真菌和细菌多样性具有显著正相关关系。随着地上植被的正向演替,土壤微生物遗传结构逐渐稳定;植被恢复早期阶段,土壤中存在着丰富的微生物遗传多样性,但并不稳定。
Based on the assumption that the genetic diversity of soil microorganisms varied with the vegetation and season, the DGGE was used to detect the genetic diversity of grassland (T), shrub (S), secondary forest (SF) and primary forest The Genetic Diversity and Seasonal Variation of Soil Bacteria and Fungous Communities in the Process of Community Succession. With succession of succession of above-ground vegetation, the bacterial community in the soil was continuous but the dominant population was not obvious. The fungal community had no continuity but obvious dominant population. Vegetation and seasons have notable effects on Shannon’s diversity of bacterial and fungal communities to varying degrees, meanwhile there are significant vegetation and seasonal interactions. There was a significant seasonal variation in Shannon’s diversity of bacterial and fungal communities in grassland soils (p <0.01), but only a significant seasonal variation in bacterial community diversity (p <0.05) in shrub soils, while diversity of bacterial and fungal communities in forest soils Sex No significant seasonal changes. Soil fungi and bacterial diversity have a significant positive correlation. With the positive succession of above-ground vegetation, the genetic structure of soil microbes gradually stabilized. In the early stage of vegetation restoration, there were abundant genetic diversity of microorganisms in soil, but they were not stable.