通过花粉管通道法将葡萄糖氧化酶基因(Glucose oxidase,GO)转入玉米自交系,并对转化后代进行了大斑病抗性鉴定。结果表明,授粉后15～20h为最佳外缘基因导入时间段,最适合的转化质粒DNA浓度为100μg·mL-1。转化获得卡那霉素抗性植株244株,PCR阳性植株13株,对转化的D0代PCR阳性植株的抗病性进行了大斑病抗性鉴定,部分转化植株的抗玉米大斑病能力有所提高,其中3株表现高抗。研究为探索大众化玉米转基因和培育抗病玉米自交系提供了新方法。 Glucose oxidase (GO) gene was transferred into maize inbred lines through pollen tube pathway, and the resistance of the transformed progenies to maculopathy was identified. The results showed that the most suitable gene transfer time was 15 ~ 20h after pollination, and the most suitable plasmid DNA concentration was 100μg · mL-1. 244 kanamycin-resistant plants and 13 PCR-positive plants were obtained by transformation, and the resistance of the transformed plants to PCR-positive D0 was identified as maculopathic Increased, of which 3 showed high resistance. The research provides a new method to explore the popularization of maize transgenes and to cultivate the resistant maize inbred lines.