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目的了解兰州地区主要四种腹泻病毒的流行病学特点。方法收集兰州大学第一医院儿科2004年7月至2005年6月5岁以下全部住院腹泻患儿400例的粪便标本,分别采用Dako公司酶免疫试剂盒检测轮状病毒、星状病毒、腺病毒,杯状病毒检测采用酶免疫法和逆转录-聚合酶链反应(RT-PCR)法。对轮状病毒、星状病毒阳性标本用RT-PCR进行毒株分型鉴定。结果400份标本中四种病毒检测阳性率依次为轮状病毒47.3%、杯状病毒15.5%、星状病毒9.5%、腺病毒7.5%。其中有混合感染的病例数占13.5%。轮状病毒毒株G血清型分型结果为G2(34.4%)、G3(32.8%)、G1(1.1%)、不同型混合感染(5.8%)、未能分型(25.9%),P基因型分型结果为P[4](45%)、P[8](22.1%)、未能分型(32.9%)。G型与P型组合P[4]G2(43.6%)、P[8]G3(25.6%),P[4]G3(13.8%)、P[8]G2(3.2%)、P[4]G1和P[8]G1各1例。星状病毒血清分型结果为1型(57.8%)、3型(2.6%)、8型(2.6%)、未能分型(36.8%)。病毒性腹泻的高发季节轮状病毒最为明显为10-12月份。发病年龄主要为2岁以下婴幼儿,轮状病毒的高发年龄是6-23月龄。结论兰州地区婴幼儿病毒性腹泻的病原复杂,轮状病毒仍是最主要病原,该年度轮状病毒的主要流行株为P[4]G2,与往年明显不同,病原混合感染比例较大,值得重视。
Objective To understand the epidemiological characteristics of four major diarrhea viruses in Lanzhou. Methods A total of 400 stool specimens from 400 children with infantile diarrhea under 5 years old from July 2004 to June 2005 were collected from the First Hospital of Lanzhou University. The rotavirus, astrovirus and adenovirus were detected by Dako enzyme immunoassay kit. , Cup virus detection by enzyme immunoassay and reverse transcription - polymerase chain reaction (RT-PCR) method. Rotavirus, astrovirus positive specimens by RT-PCR strain typing identification. Results The positive rates of four viruses in the 400 samples were as follows: rotavirus 47.3%, calicivirus 15.5%, astrovirus 9.5% and adenovirus 7.5%. The number of cases with mixed infections accounted for 13.5%. The serotypes of rotavirus strain G were G2 (34.4%), G3 (32.8%), G1 (1.1%), different mixed infection (5.8% Typing results were P [4] (45%), P [8] (22.1%), and no typing (32.9%). P [4] G3 (13.6%), P [8] G2 (3.2%), P [4] G2 (43.6%), P [8] G3 G1 and P [8] G1 each in 1 case. Astrovirus serotypes were type 1 (57.8%), type 3 (2.6%), type 8 (2.6%), and no typing (36.8%). The highest incidence of viral diarrhea rotavirus season for the most obvious 10-12 months. The age of onset is mainly infants under 2 years of age, rotavirus high age is 6-23 months old. Conclusions The pathogen of infantile virus diarrhea in Lanzhou is still complicated by rotavirus. The main epidemic strain of rotavirus in this year is P [4] G2, which is obviously different from previous years and the proportion of pathogenic mixed infections is quite large, which is worth Pay attention.