用人工接种法建立肺炎支原体感染动物模型,模拟人的发病过程,采用动物肺脏和气管做肺炎支原体分离培养,同时采用PCR技术对动物肺脏和气管标本进行检测,并用探针杂交方法作特异性分析,探讨PCR技术检测肺炎支原体感染的可行性。结果表明,从接种动物的肺脏和气管中分离出肺炎支原体,PCR检测与分离培养结果相同。PCR技术简便,快速,无交叉反应,适宜于临床推广使用。 The animal model of Mycoplasma pneumoniae infection was established by artificial inoculation method to simulate the pathogenesis of human being. Mycoplasma pneumoniae was isolated and cultured by animal lungs and trachea. Meanwhile, the lung and trachea samples were detected by PCR and analyzed by probe hybridization , To explore the feasibility of PCR detection of Mycoplasma pneumoniae infection. The results showed that mycoplasma pneumoniae was isolated from the lungs and trachea of ​​vaccinated animals, and the results of PCR detection and isolation culture were the same. PCR technology is simple, rapid, non-cross-reaction, suitable for clinical use.