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目的 观察黄参方剂改善胃癌病人症状的疗效 ,探讨该方剂诱导胃癌细胞凋亡的作用及其与一氧化氮 (NO)的关系。方法 连服黄参方剂 4周 ,治疗 38例晚期胃癌病人 ,治疗前后用TUNEL法检测胃癌标本细胞凋亡指数 ,用比色法检测胃癌组织标本、胃液和血液NO水平。所有病人在治疗前后均进行Karnofsky评分以了解近期症状改善的程度。结果 临床症状根据Karnofsky评分标准 ,患者治疗前后行为状态平均评分分别为(5 2 6 9± 2 2 13)分和 (73 2 9± 30 11)分 ,差异显著 (P <0 0 5 )。治疗前后胃癌标本细胞凋亡指数分别为 (4 5±2 0 ) %和 (11 9± 5 1) % ,差异显著 (P <0 0 5 )。治疗后血清、胃液和胃癌组织的NO水平均显著高于治疗前的NO水平 (P <0 0 1)。结论 黄参方剂能改善胃癌病人的近期症状和诱导胃癌细胞凋亡 ,这些作用的产生伴随有胃癌组织、血清和胃液的NO水平升高。
Objective To observe the efficacy of Huangshen Prescription in improving the symptoms of gastric cancer patients, and to explore the role of this prescription in inducing apoptosis of gastric cancer cells and its relationship with nitric oxide (NO). Methods Lianzao Huangshen formula was administered for 4 weeks to treat 38 patients with advanced gastric cancer. TUNEL was used to detect the apoptosis index of gastric cancer specimens before and after treatment. Colorimetric method was used to detect gastric cancer tissue samples, gastric juice and blood NO levels. Karnofsky scores were performed before and after treatment for all patients to see how recent symptoms improved. Results Clinical symptoms According to the Karnofsky scale, the mean behavioral scores of patients before and after treatment were (5 2 69 + 2 13) points and (73 2 9 ± 30 11) points, respectively, with significant differences (P <0 05). The apoptotic index of gastric cancer specimens before and after treatment was (4 5 ± 2 0)% and (11 9 ± 51)%, respectively, with significant differences (P <0 05). The levels of NO in serum, gastric juice, and gastric cancer after treatment were significantly higher than those before treatment (P < 0 01). Conclusion Huangshen prescription can improve the short-term symptoms of gastric cancer patients and induce apoptosis of gastric cancer cells. These effects are accompanied by elevated NO levels in gastric cancer tissues, serum, and gastric juice.