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目的:探讨奥美沙坦应用于糖尿病大鼠腹主动脉球囊损伤后对内膜厚度、超氧化物歧化酶(SOD)、丙二醛(MDA)含量和谷胱甘肽过氧化酶(GSH-PX)活力的的影响。方法:以SD雄性大鼠为研究对象,随机选取10只作为正常对照组(N组),余70只以链脲佐菌素(STZ)诱导建立糖尿病模型,将糖尿病大鼠随机分为糖尿病组(DM组)、糖尿病+球囊损伤假手术组(Sham组)、糖尿病+球囊损伤手术组(BI组)、糖尿病+球囊损伤手术+奥美沙坦治疗组(OM组)。5组大鼠均于术后第1天开始灌胃,OM组使用奥美沙坦,其余组用生理盐水,术后28 d取材,HE染色检测腹主动脉内膜厚度(I)、中膜厚度(M)和内膜与中膜的厚度比(I/M);试剂盒法检测血浆中SOD、MDA含量和GSH-PX活力。结果:HE染色结果示,与N组相比,DM组、Sham组血管内膜未见明显增生;与N组相比,OM组与BI组内膜可见明显增生(P<0.01);与OM组[(0.094±0.016)mm]相比,BI组内膜增生程度[(0.130±0.037)mm]较重(P<0.01)。与N组相比,其余4组SOD含量和GSH-PX活力均降低(P<0.01);与OM组比较,BI组SOD含量减少,GSH-PX活力降低(P<0.05)。与N组比较,其余4组MDA含量明显增加(P<0.01);与OM组比较,BI组MDA含量增加(P<0.01)。结论:奥美沙坦可以抑制糖尿病大鼠腹主动脉球囊损伤后再狭窄的发生,其机制可能与增加SOD含量和GSH-PX活力、减少MDA含量有关。
Objective: To investigate the effects of olmesartan on the thickness of intima, content of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH- PX) the impact of vitality. Methods: SD male rats were randomly divided into normal control group (n = 10) and the other 70 diabetic rats induced by streptozotocin (STZ). Diabetic rats were randomly divided into diabetic group (DM group), Diabetes + Sham group, Diabetes + Balloon injury group (BI group), Diabetes + Balloon injury group + Olmesartan group (OM group). The rats in the 5 groups were given gavage on the first day after the operation. Olmesartan was given to the OM group and normal saline was given to the OM group. The thickness of the intima of the abdominal aorta (I), the thickness of the intima (M) and thickness ratio of intima to media (I / M). The contents of SOD, MDA and GSH-PX in plasma were detected by kit method. Results: Compared with N group, there was no obvious hyperplasia in DM group and Sham group compared with N group. Compared with N group, OM group and BI group showed obvious hyperplasia (P <0.01) The degree of intimal hyperplasia in BI group was (0.130 ± 0.037) mm heavier than that in group [(0.094 ± 0.016) mm] (P <0.01). Compared with the N group, the SOD and GSH-PX activities in the remaining four groups were decreased (P <0.01). Compared with the OM group, the SOD content and the GSH-PX activity in the BI group decreased (P <0.05). Compared with N group, MDA content in the other four groups increased significantly (P <0.01). Compared with OM group, MDA content in BI group increased (P <0.01). Conclusion: Olmesartan can inhibit the restenosis after abdominal aorta balloon injury in diabetic rats. The mechanism may be related to the increase of SOD content, GSH-PX activity and the decrease of MDA content.