神经元逆行荧光标记物已广泛用于神经解剖学的研究。在十余种荧光标记物中,4-乙酰胺基-4′-异硫氰酰芪-2,2′-二磺酸钠盐(SITS)因具有荧光明亮、不易扩散、不被过路纤维吸收等优点,故为一种较理想的标记物。但是Payne等(1983)使用4种不同批号SITS样品后,发现只有美国Che-mical Dynamic公司(批号104157)产品才具有逆行标记神经元的作用。他们认为这种能标记的物质不是SITS本身,而是另外一种独特的荧光物质。我们在实验中发现,溶剂的pH值对SITS的逆行标记作 Neuronal retrograde fluorescent labels have been widely used in neuroanatomical studies. Among more than ten kinds of fluorescent labels, 4-acetamido-4’-isothiocyanatostilbene-2,2’-disulfonic acid sodium salt (SITS) is not fluorescent, And so on, it is a more ideal marker. However, Payne et al. (1983) used four different lot SITS samples and found that only the Che-mical Dynamic USA (Lot 104157) product had the effect of retrograde labeling of neurons. They believe that this can be marked material is not SITS itself, but another unique kind of fluorescent material. We have found in experiments that the solvent pH value for the SITS retrograde labeling is made