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选择１５例复发急性髓性白血病（ＡＭＬ）患者，研究ＢＣＬ－ＸＬ的异质性表达与复发ＡＭＬ细胞体外生长类型的关系。用白血病祖细胞培养（ＣＦＵ－Ｌ）判断生长类型，ＲＴ－ＰＣＲ方法检测 ＢＣＬ－ＸＬ ｍＲＮＡ的表达丰度。结果：ＣＦＵ－Ｌ检测中，１０例为生长型，其ＢＣＬ－ＸＬ ｍＲＮＡ ９例高表达，１例为低表达；ＣＦＵ－Ｌ检测 ５例为不生长型，ＢＣＬ－ＸＬ ｍＲＮＡ均为低表达（ Ｐ＜０．０１）。在复发ＡＭＬ中，ＢＣＬ－ＸＬ ｍＲＮＡ的高表达与其细胞体外生长类型有关，提示与其细胞的自分泌生长有关，可以作为复发ＡＭＬ再生耐药的一个判断指标。
Fifteen patients with recurrent acute myeloid leukemia (AML) were selected to study the relationship between the heterogeneity of BCL-XL and the type of in vitro growth of recurrent AML cells. The type of growth was judged by leukemic progenitor cell culture (CFU-L), and the abundance of BCL-XL mRNA was detected by RT-PCR. Results: In the CFU-L test, 10 cases were growth type. The BCL-XL mRNA was highly expressed in 9 cases and low expression in 1 case. CFU-L was detected in 5 cases as non-growth type, and BCL-XL mRNA was low P <0.01). In recurrent AML, the overexpression of BCL-XL mRNA is related to the in vitro growth of its cells, suggesting that it is related to the autocrine growth of the cells and may be used as a judgment index for the drug resistance of recurrent AML.