The geochemistry and paleogeography of the Doushantuo succession in the Shennongjia area have been insufficiently studied. Here, we report on the carbon and oxygen isotope compositions of Ediacaran Doushantuo cap carbonates from four sections(Longxi, Muyu, Yazikou and Songluo) in the Shennongjia area. A large C-isotopic gradient(~5‰) between the Longxi and Songluo sections, here identified for the first time, is inferred to have been dynamically maintained by photosynthesis in surface waters and anaerobic oxidation of dissolved organic carbon(DOC) in deep waters. Spatial variation in C-isotope chemostratigraphy among the four sections was related to Marinoan post-glacial sea-level elevation changes. At Longxi, a positive δ~(13)C_(carb) shift below the horizon containing tepee-like structure resulted from intense photosynthesis during early regression. A negative δ~(13)C_(carb) shift within the tepee-horizon was triggered by sulfate reduction and freshwater mixing with ~(13)C-depleted dissolved inorganic carbon(DIC) during late regression. A positive δ~(13)C_(carb) excursion in the uppermost part of the cap carbonate was related to enhance primary productivity and organic matter burial during early transgression. At Muyu, the carbon isotopic variation tendency, which is similar to that at Longxi, may have been mildly influenced by the surface water environment. At Songluo, the positive δ~(13)C_(carb) excursion, up to-4‰ in the lower part of the cap carbonate, was probably associated with methanogenesis in deep waters during late transgression to early regression and subsequently disappeared due to decomposition of methane hydrate during late regression. At Yazikou, the consistently stable δ~(13)C_(carb) values around-4‰ indicate that the cap carbonate may have deposited at intermediate water depths. As evidenced by diagnostic sedimentary characteristics of the study sections, the palaeogeographic framework of the Shennongjia area exhibited deepening from SE to NW during the Early Ediacaran Period in the aftermath of Snowball Earth. Here, we report on the carbon and oxygen isotope compositions of Ediacaran Doushantuo cap carbonates from four sections (Longxi, Muyu, Yazikou and Songluo) in the Shennongjia area. A large C-isotopic gradient (~ 5 ‰) between the Longxi and Songluo sections, here identified for the first time, is inferred to have been dynamically maintained by photosynthesis in surface waters and anaerobic oxidation of dissolved organic carbon (DOC) in deep waters . Spatial variation in C-isotope chemostratigraphy among the four sections was related to Marinoan post-glacial sea-level elevation changes. At Longxi, a positive δ ~ (13) C_ (carb) shift below the horizon containing tepee-like structure resulted from A negative δ ~ (13) C_ (carb) shift within the tepee-horizon was triggered by sulfate reduction and freshwater mixing with ~ (13) C-deplet A positive δ ~ (13) C_ (carb) excursion in the uppermost part of the cap carbonate was related to enhance primary productivity and organic matter burial during early transgression. At Muyu, the carbon isotone variation tendency, which is similar to that at Longxi, may have been mildly influenced by the surface water environment. At Songluo, the positive δ ~ (13) C_ (carb) excursion, up to-4 ‰ in the lower part of the cap carbonate, was probably associated with methanogenesis in deep waters during late transgression to early regression and later disappeared due to decomposition of methane hydrate during late regression. At Yazikou, the consistently stable δ ~ (13) C_ (carb) values ​​around-4 ‰ indicate that the cap carbonate may have deposited at intermediate water depths. As evidenced by diagnostic sedimentary characteristics of the study sections, the palaeogeographic framework of the Shennongjia area demonstrated deepening from S E to NW during the Early Ediacaran Period in the aftermath of Snowball Earth.