目的:探讨IL-1β对RA患者滑膜成纤维细胞（FLS）中乳腺癌耐药蛋白（BCRP/ABCG2）mRNA表达的影响，探索IL-1β对BCRP介导多药耐药性的调控作用。方法:获取RA 3例和OA 3例患者FLS，建立FLS体外培养体系，培养至Ⅴ代；①采用实时荧光定量PCR（RT-qPCR）检测RA与OA的FLS中BCRP mRNA的表达水平并比较；②评估RA病情活动度与BCRP mRNA的表达水平的相关性；③ RA-FLS分为4组，A组空白对照组，B组、C组、D组分别加入20、50、100 ng/ml的IL-1β，培养24、48、72 h，比较不同组间BCRP mRNA表达水平。定量资料采用n M（n P25，n P75）表示，两独立样本比较采用Mann-Whitney n U检验，组间比较采用Friedman检验。n 结果:① RA-FLS中BCRP mRNA表达水平高于OA组[0.68（0.26，1.16）与0.06（0.05，0.53）；n Z=-2.136，n P=0.029]。② RA-FLS的BCRP mRNA的表达量与病程、ESR、CRP及DAS28呈正相关趋势，而与发病年龄呈负相关趋势，与性别无相关性。③相同浓度IL-1β刺激不同时间RA-FLS的BCRP mRNA的表达水平比较：IL-1β浓度为0时，48 h和72 h较24 h BCRP mRNA的表达水平均升高（n χ2=6.00，n P=0.034）；IL-1β浓度为20 ng/ml（n χ2=8.00，n P=0.014）或100 ng/ml （n χ2=6.50，n P=0.040）时，72 h组BCRP mRNA的表达水平较24 h组明显升高。④不同浓度IL-1β刺激相同时间RA-FLS的BCRP mRNA的表达水平比较：72 h时，100 ng/ml组较0 ng/ml组BCRP mRNA表达升高（n χ2=12.00，n P=0.006）。n 结论:RA-FLS存在BCRP介导的耐药性，且可能与病情活动度有关。炎性细胞因子IL-1β参与调控RA-FLS的BCRP mRNA的表达，且存在时间和浓度依赖性。“,”Objective:To explore the effects of different concentrations or time points of interleukin (IL)-1β on the expression of breast cancer resistance protein (BCRP) mRNA in fibroblast-like synoviocytes (FLS) of rheumatoid arthritis (RA) patients.Methods:Synovium tissue of RA (n n=3) and osteoarthritis (OA, n n=3) patients was obtained through osteoarticular surgery and were used to establish FLS culture system n in vitro. FLS cells in generation V were used the following purposes. ① Comparing the expression levels of BCRP mRNA in RA-FLS and OA-FLS by real time quantitative polymerase chain reaction (RT-qPCR). ② Analyzing the correlation between the activity of the disease and the expression level of BCRP mRNA. ③ FLS cells were divided into A, B, C, D four groups: Group A was the control group. Group B, C, D were cocultured with low (20 ng/ml), moderate (50 ng/ml), high (100 ng/ml) concentrations of IL-1β for 24 h, 48 h, and 72 h respectively. The expression levels of BCRP mRNA in different groups were compared.n Results:① The expression of BCRP mRNA in FLS of RA patients was significantly higher than that in the OA group [0.68(0.26, 1.16) n vs 0.06(0.05, 0.53); n Z=-2.136, n P=0.029]. ② The expression of BCRP mRNA in FLS of RA patients had a positive association with the course of disease, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) and disease activity score based on 28 joint counts (DAS28), and a negative association with the age of onset, but had no correlation with gender. ③ While the concentration of IL-1β was 0 ng/ml, the expression of BCRP mRNA in the 48 h and 72 h groups was higher than that in the 24 h group (n χ2=6.00, n P=0.034). Under the condition, when the concentration of IL-1β was 20 ng/ml(n χ2=8.00, n P=0.014) or 100 ng/ml(n χ2=6.50, n P=0.040), the expression of BCRP mRNA in the 72 h group was significantly higher than that in the 24 hours group. ④ When being stimulated for 72 hours, the expression of BCRP mRNA in the 100 ng/ml group was higher than that in the 0 ng/ml group (n χ2=12.00, n P=0.006).n Conclusion:Multidrug resistance mediated by BCRP exists in RA-FLS and may be associated with disease activity. IL-1β could regulate the expression of BCRP mRNA in FLS cells of RA patients, which may be time and concentration-dependent.