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目的:采用HPLC-DAD,同时测定不同产地的10批卷柏和12批垫状卷柏中2种炔多酚(selaginellin,selaginellinB)和4种双黄酮(穗花杉双黄酮,穗花杉双黄酮-7-甲醚,扁柏双黄酮,异柳杉双黄酮)的含量。方法:采用Agilent 1100高效液相色谱系统,Waters Cosmosil C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.1%甲酸水溶液,梯度洗脱,流速1.0 mL.min-1,检测波长280,337 nm,柱温30℃。结果:所测样品中6种活性成分的量差异较大,穗花杉双黄酮在卷柏中的质量分数(5.628～9.184 mg.g-1)普遍高于垫状卷柏(0.823～7.131 mg.g-1),而selaginellin在垫状卷柏中的质量分数(0.123～0.593 mg.g-1)普遍高于卷柏(0.067～0.133 mg.g-1)。此方法加样回收率和线性范围均符合要求。结论:该方法简便快速、分离效果好、灵敏度高、重现性好,可为全面控制卷柏药材的质量提供参考。
OBJECTIVE: To determine the bioavailability of selaginellin (selaginellin, selaginellin B) and four kinds of bioflavonoids (biflavone, 7-methyl ether, cypress bflavone, isocrotophos bflavone) content. Methods: The Agilent 1100 HPLC system was used. Waters Cosmosil C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase of acetonitrile-0.1% formic acid was eluted with a gradient of 1.0 mL · min- nm, column temperature 30 ℃. Results: The content of the six active components in the tested samples varied greatly. The mass fraction of biflavonoids in Selaginella aboveground (5.628-9.184 mg.g-1) was generally higher than that in C. septentrionalis (0.823-7.131 mg). The mass fraction of selaginellin (0.123-0.593 mg.g-1) was higher than that of Selaginella (0.067-0.133 mg.g-1). This method of sample recovery rate and linear range are in line with requirements. Conclusion: The method is simple and rapid, good separation, high sensitivity and good reproducibility, which can provide reference for the comprehensive control of the quality of the medicinal materials.